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SgRNA PAM 1 PAM PAM Cas9 DNA Cas9 PAM Cas12a对靶标双链DNA的剪切位点主要取决于PAM，原有研究显示，Cas12a在非靶标链 (NTS)距PAM第18位和靶标链 (TS)距PAM第23位进行剪切，从而形成5'端5-nt悬垂的粘性末端。 但实际上，该剪切位点并不是非常专一，受到不同来源Cas12a、不同靶标序列以及不同长度crRNA的影响。
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Pam Barnhill Better TogetherCas12a（又称Cpf1）是一类由单个crRNA介导的核酸内切酶，能特异性识别并剪切带PAM（Protospacer Adjacent Motif，即原间隔区邻近基序）的双链DNA（dsDNA）靶标。 不同来源的Cas12a蛋白识别的PAM序列可能有所不同。 Cas12a家族都识别富含T的PAM序列。 PAM PAM PAM 100 150
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:max_bytes(150000):strip_icc()/Pam-Grier-Roll-Call-020623-b94fef18c4a94b97996adf141f424320.jpg "Pam Grier Reflects On Her Most Iconic Roles From Coffy To Jackie Brown")
Pam Grier Reflects On Her Most Iconic Roles From Coffy To Jackie Brown
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